Localization of kinetoplast DNA maxicircle transcripts in bloodstream and procyclic form Trypanosoma brucei.
نویسندگان
چکیده
منابع مشابه
Lipidomic analysis of bloodstream and procyclic form Trypanosoma brucei.
The biological membranes of Trypanosoma brucei contain a complex array of phospholipids that are synthesized de novo from precursors obtained either directly from the host, or as catabolised endocytosed lipids. This paper describes the use of nanoflow electrospray tandem mass spectrometry and high resolution mass spectrometry in both positive and negative ion modes, allowing the identification ...
متن کاملIsolation and characterization of kinetoplast DNA from bloodstream form of Trypanosoma brucei
We have used restriction endonucleases PstI, EcoRI, HapII, HhaI, and S1 nuclease to demonstrate the presence of a large complex component, the maxi-circle, in addition to the major mini-circle component in kinetoplast DNA (kDNA) networks of Trypanosoma brucei (East African Trypanosomiasis Research Organization [EATRO] 427). Endonuclease PstI and S1 nuclease cut the maxi-circle at a single site,...
متن کاملDepletion of mitochondrial acyl carrier protein in bloodstream-form Trypanosoma brucei causes a kinetoplast segregation defect.
Like other eukaryotes, trypanosomes have an essential type II fatty acid synthase in their mitochondrion. We have investigated the function of this synthase in bloodstream-form parasites by studying the effect of a conditional knockout of acyl carrier protein (ACP), a key player in this fatty acid synthase pathway. We found that ACP depletion not only caused small changes in cellular phospholip...
متن کاملTranscription of kinetoplast DNA in Trypanosoma brucei bloodstream and culture forms.
Kinetoplast DNA is the unusual mitochondrial DNA of trypanosomes. In Trypanosoma brucei it consists of about lo* minicircles (0.3 km) and lo* maxicircles (6 pm) catenated into a single network. The maxicircles are probably the equivalent of mitochondrial DNA in other organisms. Here we report that a fraction of the total cellular RNA from bloodstream form and culture form T. brucei hybridizes w...
متن کاملKREX2 Is Not Essential for Either Procyclic or Bloodstream Form Trypanosoma brucei
BACKGROUND Most mitochondrial mRNAs in Trypanosoma brucei require RNA editing for maturation and translation. The edited RNAs primarily encode proteins of the oxidative phosphorylation system. These parasites undergo extensive changes in energy metabolism between the insect and bloodstream stages which are mirrored by alterations in RNA editing. Two U-specific exonucleases, KREX1 and KREX2, are...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1982
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.2.7.845